Copacabana method for spreading E. By now, you should achieve even spreading of cells across the agar surface. Use glass beads that are 4 mm in diameter. Hold an inoculation loop in your right hand. Introductory Course in Microbiology.

  • Streak plate method Principle, Purpose, Procedure and results Learn Microbiology Online
  • Making a streak plate Nuffield Foundation
  • Aseptic Laboratory Techniques Plating Methods

  • A source of bacteria (stock culture, previously streaked agar plate or any Lysol (10%v/v); Agar plate (Nutrient agar or any other agar medium). In microbiology, streaking is a technique used to isolate a pure strain from a single species of The modern streak plate method has progressed from the efforts of Robert Koch and other microbiologists to obtain microbiological cultures The nutrient agar has a lot of ingredients with unknown amounts of nutrients in them.

    Streak plate method Principle, Purpose, Procedure and results Learn Microbiology Online

    Streak plates of several bacterial species on nutrient agar plates. Nutrient agar is a general purpose medium supporting growth of a wide range of non-fastidious.
    In vitro detection and primary cultivation of bacteria producing materials inhibitory to ruminal methanogens.

    The loop, stick or toothpick should never go back into the first half of the streaks in the first quadrant where most of the original inoculum was deposited.

    Video: Nutrient agar streak plates Microbiology: How to streak an agar plate

    Due to its preference for damp environments, this microorganism is commonly found growing in the corners of bathtubs, in sink basins, in tile grout, and on shower curtains. It is also used by expert practitioners to start new maintained cultures by picking off an appropriate isolated colony of an identifiable species with a sterile loop and growing the cells in sterile nutrient broth.

    The decrease of bacteria should show that colonies are sufficiently spread apart to affect the separation of the different types of microbes.

    Making a streak plate Nuffield Foundation

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    When using a turntable and glass rod, the sample may be absorbed too quickly such that the colonies form only near the center of the plate.

    Another frequent error is pouring the melted agar when it is too hot, killing many of the bacterial cells in the sample. June 30, tankeshwar Virology 3. After closing the lid, the plate should be set on the bench top undisturbed for at least 5 minutes to allow the sample to absorb completely into the agar prior to inverting the plate for incubation.

    Tape the plate closed and incubates the plate in an inverted position in an incubator for hours.

    A streak plate involves the progressive dilution of an inoculum of bacteria or with a sterile loop and growing the cells in sterile nutrient broth. The streak plate method is a rapid qualitative isolation method for obtaining dish with appropriate bacterial media(such as trypticase soy agar, nutrient agar). With the streak-plate procedure, a mixture of cells is spread over the surface of a semi-solid, agar-based nutrient medium in a Petri dish such.
    After gently swirling to mix, the plate was set upon a flat surface and the agar was allowed to solidify completely.

    If you do touch the tip to another surface or blow on it, you will have to re-flame the loop before you proceed to your experiment.

    Aseptic Laboratory Techniques Plating Methods

    If there is more than one type of colony, each type should be streaked again on a separate plate to obtain a pure culture. In either case, the results will be ambiguous or unreadable at best. As soon as the inoculation is completed, flame your loop or needle. However, some lytic phage produce interesting patterns such as the "bull's eye" plaque shown in Figure 12B.

    In microbiologystreaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria.

    Nutrient agar streak plates
    Example result using replica-plate procedure.

    Biofilm involves mixed microbial populations. Thats good work but sir plz you have just mentioned the referance of image but not for other material???? Line up the orientation mark on the plate with the mark on the block. Then the plate is placed on a level surface until the top agar layer has had time to solidify and subsequently can be placed in the incubator.

    Prepare a nutrient agar plate (see Appendix medium no.

    3) and streak your plate with your unknown bacteria to isolate single colonies.

    After incubating the plate. media (up to ca 10 cm3); making streak plates x Take a nutrient agar pour or spread/lawn plate of e.g.

    images nutrient agar streak plates

    Bacillus subtilis, Micrococcus luteus, Escherichia coli.
    The last plate in the series was a positive control YTA plate E. Move the loop, stick, or toothpick back and forth many times across the agar surface from the rim to the center of the plate.

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    images nutrient agar streak plates

    Introductory Course in Microbiology. Replica Plate Procedure: Transfer of Cells for Screening Mutants and Auxotrophs This technique permits comparison of cell growth on a primary plate to secondary plates, generating a means to screen cells for a selectable phenotype.

    Do not let the loop touch any of the previously streaked areas.

    Video: Nutrient agar streak plates Streak Plate Technique Microbiology 2016

    Nutrient agar streak plates
    The ethanol must only touch the bottom portion of the spreader and the first inch of the stem. At the end of incubation there should be enough bacteria to form visible colonies in the areas touched by the inoculation loop.

    Example of single colonies on a plate. The procedure is then repeated once more being cautious to not touch the previously streaked sectors. Open the lid of the empty Petri dish, and dispense your sample into the middle of the plate Panel A of Figure 4.

    images nutrient agar streak plates


    1. Wooden sticks are transferred to 18 mm test tubes then autoclaved to sterilize before use. Center the plate on the turntable Figure 5.

    2. Do you have any queries?

    3. Multiple plates may be stacked and taped together then inverted for incubation.